Application Note

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Polyphenols are widely recognized as functional components of food and beverage products, such as tea, wine, and fruits. Polyphenols are reported to play a potential role in protecting human health from degenerative and cardiovascular diseases.

Over the years, the determination of natural phenolic compounds has proved an extremely difficult analytical challenge, which has been hindered by the complexity of the numerous chemical classes and species, combined with inherent limitations of conventional HPLC separation methods. Typical HPLC methods that are used to separate and identify polyphenolic compounds by reversed phrase chromatography often take more than an hour for a single run.

More recently, analysis methods of polyphenols using Waters'® ACQUITY UPLC System have developed, which provide increased resolution and shortened analysis times. The reduction of run time enables and increase sample throughput, which is crucial for juice QC laboratories to deliver their products to store shelves more quickly.

Waters ACQUITY UPLC H-Class System enables routine HPLC users to move to UPLC Technology easily by combining the speed and performance of UPLC with the ability to run HPLC separations. The use of the ACQUITY H-Class with UV and mass spectrometry provides a robust solution for the QC analysis of juice samples.

ACQUITY UPLC H-Class System Benefits

• Rapidly separate, detect, identify, and quantify polyphenolic compounds in fruit juices.
• Reduces analysis time from over and hour with HPLC methods to fewer than 10 minutes using ACQUITY UPLC, increasing sample throughout for juice QC labs along with improved chromatographic performance.
• Allows easy access to UV and mass spectral information for analytical labs performing quantification, data interpretation, and compound identification.

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